Because the depth of field of the high-magnification objectives is shallow and the tissue sample is not always flat, continuous refocusing is required to retain a clear view. Since the region is extremely small, the pathologist needs to maneuver the stage that carries the glass slide translationally to move from one region to another. Using a light microscope, the pathologist examines a tiny region of the glass slide that is magnified by the objectives and eye pieces. Thanks to technological advances in digital microscopy, image compression algorithms, display quality, computer performance, and Internet connectivity, the whole-slide imaging (WSI) scanner is emerging as a replacement for the light microscope that has been used for decades by pathologists to review tissue slides.
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